Identification of Partially Disordered Peptide Intermediates through Residue-Specific NMR Diffusion Measurements

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• 作者：Yingjie Li ; Seho Kim ; Barbara Brodsky ; and Jean Baum
• 刊名：Journal of the American Chemical Society
• 出版年：2005
• 出版时间：August 3, 2005
• 年：2005
• 卷：127
• 期：30
• 页码：10490 - 10491
• 全文大小：99K
• 年卷期：v.127,no.30(August 3, 2005)
• ISSN：1520-5126

文摘

It is technically challenging to detect low-population partially disordered species that are in equilibrium with the folded and unfolded states. Residue-specific translational diffusion experiments measured by pulsed field gradient NMR have been used to detect the presence, and define the conformation, of such equilibrium intermediates. The experiment is demonstrated for equilibrium solutions of related triple helical peptides that model a small region of type I collagen with and without a mutation known to cause osteogenesis imperfecta. The data show that residue-specific diffusion coefficients of an interconverting trimer to monomer system can allow discrimination between a simple two-state model and more complex multistate models involving partially disordered intermediates.