Fast, Ultrasensitive Detection of Reactive Oxygen Species Using a Carbon Nanotube Based-Electrocatalytic Intracellular Sensor

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• 作者：Frankie J. Rawson ; Jacqueline Hicks ; Nicholas Dodd ; Wondwossen Abate ; David J. Garrett ; Nga Yip ; Gyorgy Fejer ; Alison J. Downard ; Kim H. R. Baronian ; Simon K. Jackson ; Paula M. Mendes
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2015
• 出版时间：October 28, 2015
• 年：2015
• 卷：7
• 期：42
• 页码：23527-23537
• 全文大小：631K
• ISSN：1944-8252

文摘

Herein, we report a highly sensitive electrocatalytic sensor-cell construct that can electrochemically communicate with the internal environment of immune cells (e.g., macrophages) via the selective monitoring of a particular reactive oxygen species (ROS), hydrogen peroxide. The sensor, which is based on vertically aligned single-walled carbon nanotubes functionalized with an osmium electrocatalyst, enabled the unprecedented detection of a local intracellular 鈥減ulse鈥?of ROS on a short second time scale in response to bacterial endotoxin (lipopolysaccharide-LPS) stimulation. Our studies have shown that this initial pulse of ROS is dependent on NADPH oxidase (NOX) and toll like receptor 4 (TLR4). The results suggest that bacteria can induce a rapid intracellular pulse of ROS in macrophages that initiates the classical innate immune response of these cells to infection.