Stimuli-Responsive Polymer-Antibody Conjugates via RAFT and Tetrafluorophenyl Active Ester Chemistry

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• 作者：Debashish Roy ; Barrett J. Nehilla ; James J. Lai ; Patrick S. Stayton
• 刊名：ACS Macro Letters
• 出版年：2013
• 出版时间：February 19, 2013
• 年：2013
• 卷：2
• 期：2
• 页码：132-136
• 全文大小：260K
• 年卷期：v.2,no.2(February 19, 2013)
• ISSN：2161-1653

文摘

Highly efficient polymer-antibody conjugations were demonstrated via a tetrafluorophenyl active ester. A well-defined diblock copolymer was synthesized by reversible addition鈥揻ragmentation chain transfer (RAFT) polymerization with a temperature-responsive block, poly(N-isopropylacrylamide), and a block of N,N-dimethylacrylamide and 2,3,5,6-tetrafluorophenyl acrylate active ester. The polymer was conjugated to anti-p24 IgG antibody with about 100% efficiency in as little as 2 h at room temperature in a pH 10.8 buffer. The temperature-responsiveness of the polymer was conferred to the polymer鈥揳ntibody conjugates after conjugation. The conjugates bound p24 antigen specifically and with binding efficiency comparable to native antibodies. Thus, the active ester diblock copolymer can facilitate the synthesis of temperature-responsive bioconjugates, which may be promising reagents for immunoassays, bioseparations, and specimen-enrichment applications.