Evaluation of Fatty Acid Extraction Methods for Thraustochytrium sp. ONC-T18

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• 作者：Adam M. Burja ; Roberto E. Armenta ; Helia Radianingtyas ; Colin J. Barrow
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2007
• 出版时间：June 13, 2007
• 年：2007
• 卷：55
• 期：12
• 页码：4795 - 4801
• 全文大小：489K
• 年卷期：v.55,no.12(June 13, 2007)
• ISSN：1520-5118

文摘

Various extraction methods were assessed in their capacity to extract fatty acids from a dried biomassof Thraustochytrium sp. ONC-T18. Direct saponification using KOH in ethanol or in hexane:ethanolwas one of the most efficient techniques to extract lipids (697 mg g^-1). The highest amount of fattyacids (714 mg g^-1) was extracted using a miniaturized Bligh and Dyer extraction technique. The useof ultrasonics to break down cell walls while extracting with solvents (methanol:chloroform) also offeredhigh extraction yields of fatty acids (609 mg g^-1). Moreover, when the transesterification mixtureused for a direct transesterification method was doubled, the extraction of fatty acids increasedapproximately 77% (from 392 to 696 mg g^-1). This work showed that Thraustochytrium sp. ONC-T18 has the ability to produce over 700 mg g^-1 of lipids, including more than 165 mg g^-1 ofdocosahexaenoic acid, which makes this microorganism a potential candidate for the commercialproduction of polyunsaturated fatty acids. Finally, other lipids, such as myristic, palmitic, palmitoleic,and oleic acids, were also produced and recovered in significant amounts (54, 196, 123, and 81 mgg^-1), respectively.