Identification of Major Dioxin-Like Compounds and Androgen Receptor Antagonist in Acid-Treated Tissue Extracts of High Trophic-Level Animals

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• 作者：Go Suzuki ; Nguyen M. Tue ; Sander van der Linden ; Abraham Brouwer ; Bart van der Burg ; Martin van Velzen ; Marja Lamoree ; Masayuki Someya ; Shin Takahashi ; Tomohiko Isobe ; Yuko Tajima ; Tadasu K. Yamada ; Hidetaka Takigami ; Shinsuke Tanabe
• 刊名：Environmental Science & Technology
• 出版年：2011
• 出版时间：December 1, 2011
• 年：2011
• 卷：45
• 期：23
• 页码：10203-10211
• 全文大小：993K
• 年卷期：v.45,no.23(December 1, 2011)
• ISSN：1520-5851

文摘

We evaluated the applicability of combining in vitro bioassays with instrument analyses to identify potential endocrine disrupting pollutants in sulfuric acid-treated extracts of liver and/or blubber of high trophic-level animals. Dioxin-like and androgen receptor (AR) antagonistic activities were observed in Baikal seals, common cormorants, raccoon dogs, and finless porpoises by using a panel of rat and human cell-based chemical-activated luciferase gene expression (CALUX) reporter gene bioassays. On the other hand, no activity was detected in estrogen receptor 伪 (ER伪)-, glucocorticoid receptor (GR)-, progesterone receptor (PR)-, and peroxisome proliferator-activated receptor 纬2 (PPAR纬2)-CALUX assays with the sample amount applied. All individual samples (n = 66) showed dioxin-like activity, with values ranging from 21 to 5500 pg CALUX-2,3,7,8-tetrachlorodibenzo-p-dioxin equivalent (TEQ)/g-lipid. Because dioxins are expected to be strong contributors to CALUX-TEQs, the median theoretical contribution of dioxins calculated from the result of chemical analysis to the experimental CALUX-TEQs was estimated to explain up to 130% for all the tested samples (n = 54). Baikal seal extracts (n = 31), but not other extracts, induced AR antagonistic activities that were 8鈥?50 渭g CALUX-flutamide equivalent (FluEQ)/g-lipid. p,p鈥?/i>-DDE was identified as an important causative compound for the activity, and its median theoretical contribution to the experimental CALUX-FluEQs was 59% for the tested Baikal seal tissues (n = 25). Our results demonstrate that combining in vitro CALUX assays with instrument analysis is useful for identifying persistent organic pollutant-like compounds in the tissue of wild animals on the basis of in vitro endocrine disruption toxicity.