A Structured Chitosan-Based Platform for Biomolecule Attachment to Solid Surfaces: Application to DNA Microarray Preparation
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- 作者：Clarissa Consolandi ; Marco Severgnini ; Bianca Castiglioni ; Roberta Bordoni ; Andrea Frosini ; Cristina Battaglia ; Luigi Rossi Bernardi ; and Gianluca De Bellis
- 刊名：Bioconjugate Chemistry
- 出版年：2006
- 出版时间：March 2006
- 年：2006
- 卷：17
- 期：2
- 页码：371 - 377
- 全文大小：154K
- 年卷期：v.17,no.2(March 2006)
- ISSN：1520-4812
文摘
A structured chemical platform based on chitosan, an amine-rich polysaccharide, is presented as an alternativechemistry to functionalize solid support (in this case, glass slides) for grafting biomolecules. This approach hasbeen adopted for generating arrays using amino-modified oligonucleotides with two different lengths (25-merand 70-mer) for different purposes. Results using these chitosan-activated surfaces indicate high oligonucleotideloading capacity, good availability to hybridization against targets, and effectiveness in enzyme-mediated singlenucleotide polymorphism (SNP) detection procedures by DNA polymerase and DNA ligase enzymes with lowbackground. Universal arrays have been prepared and extensively used with excellent results in differentapplications. The chitosan-treated surfaces were also evaluated for their performance in a gene expressionexperiment.