Continuous Flow Ion Mobility Separation with Mass Spectrometric Detection Using a Nano-Radial Differential Mobility Analyzer at Low Flow Rates

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• 作者：N. A. Brunelli ; E. L. Neidholdt ; K. P. Giapis ; R. C. Flagan ; J. L. Beauchamp
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：May 7, 2013
• 年：2013
• 卷：85
• 期：9
• 页码：4335-4341
• 全文大小：377K
• 年卷期：v.85,no.9(May 7, 2013)
• ISSN：1520-6882

文摘

We describe a hybrid mass-mobility instrument in which a continuous-flow ion mobility classifier is used as a front-end separation device for mass spectrometric analysis of ions generated with an electrospray ionization source. Using nitrogen as a carrier gas, the resolving power of the nano-radial differential mobility analyzer (nRDMA) for nanometer-sized ions is 5鈥? for tetraalkylammonium ions. Data are presented demonstrating the ability of the system to resolve the different aggregation and charge states of tetraalkylammonium ions and protonated peptides using a quadrupole ion trap (QIT) mass spectrometer to analyze the mobility-classified ions. Specifically, data are presented for the two charge states of the decapeptide Gramicidin S. A key feature of the new instrument is the ability to continuously transmit ions with specific mobilities to the mass spectrometer for manipulation and analysis.