Nano-Chemical Infrared Imaging of Membrane Proteins in Lipid Bilayers
详细信息 查看全文
- 作者：Samuel Berweger ; Duc M. Nguyen ; Eric A. Muller ; Hans A. Bechtel ; Thomas T. Perkins ; Markus B. Raschke
- 刊名：Journal of the American Chemical Society
- 出版年：2013
- 出版时间：December 11, 2013
- 年：2013
- 卷：135
- 期：49
- 页码：18292-18295
- 全文大小：372K
- 年卷期：v.135,no.49(December 11, 2013)
- ISSN：1520-5126
文摘
The spectroscopic characterization of biomolecular structures requires nanometer spatial resolution and chemical specificity. We perform full spatio-spectral imaging of dried purple membrane patches purified from Halobacterium salinarum with infrared vibrational scattering-type scanning near-field optical microscopy (s-SNOM). Using near-field spectral phase contrast based on the Amide I resonance of the protein backbone, we identify the protein distribution with 20 nm spatial resolution and few-protein sensitivity. This demonstrates the general applicability of s-SNOM vibrational nanospectroscopy, with potential extension to a wide range of biomolecular systems.