Evidence that Norflurazon Affects Chloroplast Lipid Unsaturation in Soybean Leaves (Glycine max L.)

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• 作者：Ouzna Abrous-Belbachir ; Rosine De Paepe ; Antoine Trmolires ; Chantal Mathieu ; Fatiha Ad ; Ghouziel Benhassaine-Kesri
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2009
• 出版时间：December 9, 2009
• 年：2009
• 卷：57
• 期：23
• 页码：11434-11440
• 全文大小：798K
• 年卷期：v.57,no.23(December 9, 2009)
• ISSN：1520-5118

文摘

Norflurazon is a bleaching herbicide known to block carotenoid biosynthesis by inhibiting phytoene desaturase activity. Soybean plants were treated with norflurazon, and we examined the effects on the desaturation of lipid molecular species in leaves using ammonium [1-¹⁴C] oleate labeling. In monogalactosyldiacylglycerol (MGDG), the main chloroplast lipid, a decrease in 18:3/18:3 molecular species and an increase in its precursors 18:2/18:3 and 18:2/18:2 were observed suggesting that the ω³ FAD7 desaturase activity in planta was inhibited by norflurazon. The in vitro activity of MGDG synthase was also inhibited by 69%. In contrast, the amount of 18:3/18:3 molecular species of phosphatidylcholine (PC) in the extraplastid compartment increased. The observed increase in in vitro lysoPC-acyltransferase activity and activation of desaturation of [1-¹⁴C] oleate suggest that extraplastid ω³FAD3 desaturase was activated. Analysis of the expression of ω³ FAD3 and ω³ FAD7 genes in norflurazon treated plants indicate that ω³ FAD7 and ω³ FAD3 desaturases are controlled at the post-transcriptional level.