Near-IR Two-Photon Fluorescent Sensor for K+ Imaging in Live Cells

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• 作者：Binglin Sui ; Xiling Yue ; Bosung Kim ; Kevin D. Belfield
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2015
• 出版时间：August 19, 2015
• 年：2015
• 卷：7
• 期：32
• 页码：17565-17568
• 全文大小：264K
• ISSN：1944-8252

文摘

A new two-photon excited fluorescent K⁺ sensor is reported. The sensor comprises three moieties, a highly selective K⁺ chelator as the K⁺ recognition unit, a boron-dipyrromethene (BODIPY) derivative modified with phenylethynyl groups as the fluorophore, and two polyethylene glycol chains to afford water solubility. The sensor displays very high selectivity (>52-fold) in detecting K⁺ over other physiological metal cations. Upon binding K⁺, the sensor switches from nonfluorescent to highly fluorescent, emitting red to near-IR (NIR) fluorescence. The sensor exhibited a good two-photon absorption cross section, 500 GM at 940 nm. Moreover, it is not sensitive to pH in the physiological pH range. Time-dependent cell imaging studies via both one- and two-photon fluorescence microscopy demonstrate that the sensor is suitable for dynamic K⁺ sensing in living cells.