pH-Degradable Mannosylated Nanogels for Dendritic Cell Targeting

详细信息    查看全文

• 作者：Ruben De Coen ; Nane Vanparijs ; Martijn D. P. Risseeuw ; Lien Lybaert ; Benoit Louage ; Stefaan De Koker ; Vimal Kumar ; Johan Grooten ; Leeanne Taylor ; Neil Ayres ; Serge Van Calenbergh ; Lutz Nuhn ; Bruno G. De Geest
• 刊名：Biomacromolecules
• 出版年：2016
• 出版时间：July 11, 2016
• 年：2016
• 卷：17
• 期：7
• 页码：2479-2488
• 全文大小：584K
• 年卷期：0
• ISSN：1526-4602

文摘

We report on the design of glycosylated nanogels via core-cross-linking of amphiphilic non-water-soluble block copolymers composed of an acetylated glycosylated block and a pentafluorophenyl (PFP) activated ester block prepared by reversible addition–fragmentation (RAFT) polymerization. Self-assembly, pH-sensitive core-cross-linking, and removal of remaining PFP esters and protecting groups are achieved in one pot and yield fully hydrated sub-100 nm nanogels. Using cell subsets that exhibit high and low expression of the mannose receptor (MR) under conditions that suppress active endocytosis, we show that mannosylated but not galactosylated nanogels can efficiently target the MR that is expressed on the cell surface of primary dendritic cells (DCs). These nanogels hold promise for immunological applications involving DCs and macrophage subsets.