Aptamer-Based Viability Impedimetric Sensor for Viruses
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- 作者：Mahmoud Labib ; Anna S. Zamay ; Darija Muharemagic ; Alexey V. Chechik ; John C. Bell ; Maxim V. Berezovski
- 刊名：Analytical Chemistry
- 出版年：2012
- 出版时间：February 21, 2012
- 年：2012
- 卷：84
- 期：4
- 页码：1813-1816
- 全文大小：240K
- 年卷期：v.84,no.4(February 21, 2012)
- ISSN：1520-6882
文摘
The development of aptamer-based viability impedimetric sensor for viruses (AptaVISens-V) is presented. Highly specific DNA aptamers to intact vaccinia virus were selected using cell-SELEX technique and integrated into impedimetric sensors via self-assembly onto a gold microelectrode. Remarkably, this aptasensor is highly selective and can successfully detect viable vaccinia virus particles (down to 60 virions in a microliter) and distinguish them from nonviable viruses in a label-free electrochemical assay format. It also opens a new venue for the development of a variety of viability sensors for detection of many microorganisms and spores.