Four-Way Junction Formation Promoting Ultrasensitive Electrochemical Detection of MicroRNA
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- 作者：Mahmoud Labib ; Shahrokh M. Ghobadloo ; Nasrin Khan ; Dmitry M. Kolpashchikov ; Maxim V. Berezovski
- 刊名：Analytical Chemistry
- 出版年：2013
- 出版时间：October 15, 2013
- 年：2013
- 卷：85
- 期：20
- 页码：9422-9427
- 全文大小：334K
- 年卷期：v.85,no.20(October 15, 2013)
- ISSN：1520-6882
文摘
MicroRNAs (miRNAs) represent a class of biomarkers that are frequently deregulated in cancer cells and have shown a great promise for cancer classification and prognosis. Here, we endeavored to develop a DNA four-way junction based electrochemical sensor (4J-SENS) for ultrasensitive miRNA analysis. The developed sensor can be operated within the dynamic range from 10 aM to 1 fM and detect as low as 2 aM of miR-122 (36 molecules per sample), without PCR amplification. Furthermore, the 4J-SENS was employed to profile endogenouse hsa-miR-122 in healthy human and chronic lymphocyitc leukemia (CLL) patient serum, and the results were validated by qPCR analysis.