Enzyme-Linked Immunosorbent Assay-Based Detection of Free Trenbolone in Bovine Bile

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• 作者：Jane Fitzpatrick ; Bernadette Manning ; and Richard O'Kennedy
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2004
• 出版时间：July 14, 2004
• 年：2004
• 卷：52
• 期：14
• 页码：4351 - 4354
• 全文大小：55K
• 年卷期：v.52,no.14(July 14, 2004)
• ISSN：1520-5118

文摘

A rapid antibody-based detection system has been developed for the presence of free trenbolone inbovine samples. Polyclonal antibodies were produced that showed specificity toward epitopes locatedaround the steroidal A-ring of the trenbolone molecule. These antibodies were shown to have little orno recognition for many closely related compounds. The antibodies were utilized as the specificbiorecognition molecules in competitive and inhibitive enzyme-linked immunosorbent assay systems.While both assays were able to detect low nanogram concentrations of trenbolone in bovine bile, thecompetitive format was more sensitive (2.41 vs 17.15 ng/mL for TRAb2 and 3.31 vs 30.73 ng/mL forTRAb1). This format was also more accurate and the data produced by this assay fitted more closelyto the four parameter equation used to calculate the standard curve. This was a common findingwith both of the polyclonal antibodies, suggesting that this was a characteristic of the format used.Keywords: Trenbolone; ELISA; bovine bile