Assessment of IgE and IgG4 Binding Capacities of Cow’s Milk Proteins Selectively Altered by Proteases

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• 作者：Alexandre Charcosset ; Karine Adel-Patient ; Christophe Dupont ; Hervé Bernard
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：May 4, 2016
• 年：2016
• 卷：64
• 期：17
• 页码：3394-3404
• 全文大小：479K
• 年卷期：0
• ISSN：1520-5118

文摘

Specific IgE and IgG4 have been reported to play key roles in the context of IgE-mediated cow’s milk allergy (CMA), but the persistence of their epitopes in milk hydrolysates has not been evaluated. Using sera from 32 CMA patients, 6 CMA patients treated by epicutaneous therapy (CM-treated), and 4 CM-tolerant peanut allergic patients, we analyzed the IgE and IgG4 binding capacities related to major milk allergens in processed milk. Different proteases (plasmin, chymosin, α-chymotrypsin, or pepsin) were used progressively and selectively to hydrolyze β-lactoglobulin (β-LG) and casein (CN) in milk. We then showed that proteases differentially affect IgE or IgG4 immunoreactivities of CN and β-LG and also that we could not relate IgE and/or IgG4 levels or specificities to milk hydrolysates to the clinical status of the patients.