In Vivo Characterization of Atherosclerotic Plaque Depositions by Raman-Probe Spectroscopy and in Vitro Coherent Anti-Stokes Raman Scattering Microscopic Imaging on a Rabbit Model

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• 作者：Christian Matth盲us ; Sebastian Dochow ; Gero Bergner ; Annika Lattermann ; Bernd F. M. Romeike ; Eric T. Marple ; Christoph Krafft ; Benjamin Dietzek ; Bernhard R. Brehm ; J眉rgen Popp
• 刊名：Analytical Chemistry
• 出版年：2012
• 出版时间：September 18, 2012
• 年：2012
• 卷：84
• 期：18
• 页码：7845-7851
• 全文大小：389K
• 年卷期：v.84,no.18(September 18, 2012)
• ISSN：1520-6882

文摘

Visualization as well as characterization of inner arterial plaque depositions is of vital diagnostic interest, especially for the early recognition of vulnerable plaques. Established clinical techniques provide valuable visual information but cannot deliver information about the chemical composition of individual plaques. Here, we employ Raman-probe spectroscopy to characterize the plaque compositions of arterial walls on a rabbit model in vivo, using a miniaturized filtered probe with one excitation and 12 collection fibers integrated in a 1 mm sleeve. Rabbits were treated with a cholesterol-enriched diet. The methodology can improve the efficiency of animal experiments and shows great potential for applications in cardiovascular research. In order to further characterize the plaque depositions visually, coherent anti-Stokes Raman scattering (CARS) microscopy images have been acquired and are compared with the Raman-probe results.