Selective Chemical Imaging of Static Actin in Live Cells
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- 作者：Lech-Gustav Milroy ; Stefano Rizzo ; Abram Calderon ; Bernhard Ellinger ; Silke Erdmann ; Justine Mondry ; Peter Verveer ; Philippe Bastiaens ; Herbert Waldmann ; Leif Dehmelt ; Hans-Dieter Arndt
- 刊名：The Journal of the American Chemical Society
- 出版年：2012
- 出版时间：May 23, 2012
- 年：2012
- 卷：134
- 期：20
- 页码：8480-8486
- 全文大小：474K
- 年卷期：v.134,no.20(May 23, 2012)
- ISSN：1520-5126
文摘
We have characterized rationally designed and optimized analogues of the actin-stabilizing natural products jasplakinolide and chondramide C. Efficient actin staining was achieved in fixed permeabilized and non-permeabilized cells using different combinations of dye and linker length, thus highlighting the degree of molecular flexibility of the natural product scaffold. Investigations into synthetically accessible, non-toxic analogues have led to the characterization of a powerful cell-permeable probe to selectively image static, long-lived actin filaments against dynamic F-actin and monomeric G-actin populations in live cells, with negligible disruption of rapid actin dynamics.