Metabolism of the Fusarium Mycotoxins T-2 Toxin and HT-2 Toxin in Wheat
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- 作者：Alexis V. Nathanail ; Elisabeth Varga ; Jacqueline Meng-Reiterer ; Christoph Bueschl ; Herbert Michlmayr ; Alexandra Malachova ; Philipp Fruhmann ; Marika Jestoi ; Kimmo Peltonen ; Gerhard Adam ; Marc Lemmens ; Rainer Schuhmacher ; Franz Berthiller
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2015
- 出版时间：September 9, 2015
- 年：2015
- 卷：63
- 期：35
- 页码：7862-7872
- 全文大小：568K
- ISSN：1520-5118
文摘
To investigate the metabolic fate of HT-2 toxin (HT2) and T-2 toxin (T2) in wheat (Triticum aestivum L.), an untargeted metabolomics study utilizing stable isotopic labeling and liquid chromatography鈥揾igh resolution mass spectrometry was performed. In total, 11 HT2 and 12 T2 derived in planta biotransformation products were annotated putatively. In addition to previously reported mono- and diglucosylated forms of HT2, evidence for the formation of HT2-malonyl-glucoside and feruloyl-T2, as well as acetylation and deacetylation products in wheat was obtained for the first time. To monitor the kinetics of metabolite formation, a time course experiment was conducted involving the Fusarium head blight susceptible variety Remus and the resistant cultivar CM-82036. Biotransformation reactions were observed already at the earliest tested time point (6 h after treatment), and formed metabolites showed different kinetic profiles. After ripening, less than 15% of the toxins added to the plants were determined to be unmetabolized.