Humanization of Lepidopteran Insect-Cell-Produced Glycoproteins
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- 作者：Noboru Tomiya ; Michael J. Betenbaugh ; and Yuan C. Lee
- 刊名：Accounts of Chemical Research
- 出版年：2003
- 出版时间：August 2003
- 年：2003
- 卷：36
- 期：8
- 页码：613 - 620
- 全文大小：215K
- 年卷期：v.36,no.8(August 2003)
- ISSN：1520-4898
文摘
The insect cell-baculovirus expression vector system, widely usedfor glycoprotein production, is not ideal for pharmaceutical glycoprotein production due to the characteristics of the N-glycansin the expressed products. Insect cells lack several enzymesrequired for mammalian-type N-glycan synthesis and contain aspecific N-acetylglucosaminidase that stunts the growth of chainsand a core 
-1,3-fucosyltransferase that yields potentially allergenicglycoforms. Current knowledge on N-glycan processing in lepidopteran insect cells is summarized, and strategies to developbetter glycoprotein expression systems suitable for pharmaceuticalglycoprotein production are discussed.
-1,3-fucosyltransferase that yields potentially allergenicglycoforms. Current knowledge on N-glycan processing in lepidopteran insect cells is summarized, and strategies to developbetter glycoprotein expression systems suitable for pharmaceuticalglycoprotein production are discussed.