Size of Supramolecular SNARE Complex: Membrane-Directed Self-Assembly

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• 作者：Won Jin Cho ; Aleksandar Jeremic ; and Bhanu P. Jena
• 刊名：Journal of the American Chemical Society
• 出版年：2005
• 出版时间：July 27, 2005
• 年：2005
• 卷：127
• 期：29
• 页码：10156 - 10157
• 全文大小：224K
• 年卷期：v.127,no.29(July 27, 2005)
• ISSN：1520-5126

文摘

Full length v-SNARE protein in lipid vesicles when exposed to t-SNARE-reconstituted lipid membrane results in the self-assembly of a t-/v-SNARE complex in a ring pattern, forming pores, and establishing continuity between the opposing bilayers. It is known that smaller vesicles fuse more efficiently than larger ones, and hence the curvature of secretory vesicles may dictate the potency and efficacy of their fusion at the cell plasma membrane. The diameter of t- and v-SNARE vesicles may, therefore, reflect the size of the t-/v-SNARE complex formed. In the present study, this hypothesis was tested, and results from the study demonstrate that the size of the t-/v-SNARE complex is directly proportional to the vesicle diameter (R² = 0.9725).