Membrane Lipids Influence Protein Complex Assembly−Disassembly

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• 作者：Leah Shin ; Won Jin Cho ; Jeremy D. Cook ; Timothy L. Stemmler ; Bhanu P. Jena
• 刊名：Journal of the American Chemical Society
• 出版年：2010
• 出版时间：April 28, 2010
• 年：2010
• 卷：132
• 期：16
• 页码：5596-5597
• 全文大小：185K
• 年卷期：v.132,no.16(April 28, 2010)
• ISSN：1520-5126

文摘

Approximately 11% smaller t-/v-SNARE ring complexes are generated using 50 nm cholesterol-associated vesicles as opposed to vesicles containing pan class="smallcaps">lpan>-α-lysophosphatidylcholine (LPC), as observed using atomic force microscopy. Circular dichroism spectroscopy demonstrated that in the presence of LPC as opposed to cholesterol, N-ethylmaleimide-sensitive factor + adenosine triphosphate induces disassembly of β-sheet structures but not the α-helical contents within the t-/v-SNARE complex.