Quadruplex Formation by a Guanine-Rich PNA Oligomer

详细信息    查看全文

• 作者：Bhaskar Datta ; Mark E. Bier ; Subhadeep Roy ; and Bruce A. Armitage
• 刊名：Journal of the American Chemical Society
• 出版年：2005
• 出版时间：March 30, 2005
• 年：2005
• 卷：127
• 期：12
• 页码：4199 - 4207
• 全文大小：251K
• 年卷期：v.127,no.12(March 30, 2005)
• ISSN：1520-5126

文摘

A guanine-rich PNA dodecamer having the sequence H-G₄T₄G₄-Lys-NH₂ (G₄-PNA) hybridizeswith a DNA dodecamer of homologous sequence to form a four-stranded quadruplex (Datta, B.; Schmitt,C.; Armitage, B. A. J. Am. Chem. Soc. 2003, 125, 4111-4118). This report describes quadruplex formationby the PNA alone. UV melting curves and fluorescence resonance energy transfer experiments revealformation of a multistranded structure stabilized by guanine tetrads. The ion dependency of these structuresis analogous to that reported for DNA quadruplexes. Electrospray ionization mass spectrometry indicatesthat both dimeric and tetrameric quadruplexes are formed by G₄-PNA, with the dimeric form being preferred.These results have implications for the use of G-rich PNA for homologous hybridization to G-rich targetsin chromosomal DNA and suggest additional applications in assembling quadruplex structures within lipidbilayer environments.