Intermittent Fluorescence Oscillations in Lipid Droplets in a Live Normal and Lung Cancer Cell: Time-Resolved Confocal Microscopy

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• 作者：Rajdeep Chowdhury ; Md. Asif Amin ; Kankan Bhattacharyya
• 刊名：Journal of Physical Chemistry B
• 出版年：2015
• 出版时间：August 27, 2015
• 年：2015
• 卷：119
• 期：34
• 页码：10868-10875
• 全文大小：524K
• ISSN：1520-5207

文摘

Intermittent structural oscillation in the lipid droplets of live lung cells is monitored using time-resolved confocal microscopy. Significant differences are observed between the lung cancer cell (A549) and normal (nonmalignant) lung cell (WI38). For this study, the lipid droplets are covalently labeled with a fluorescent dye, coumarin maleimide (7-diethylamino-3-(4-maleimido-phenyl)-4-methylcoumarin, CPM). The number of lipid droplets in the cancer cell is found to be 鈭?0-fold higher than that in the normal (nonmalignant) cell. The fluctuation in the fluorescence intensity of the dye (CPM) is attributed to the red鈥搊x processes and periodic formation/rupture of the S鈥揅PM bond. The amount of reactive oxygen species (ROS) is much higher in a cancer cell. This is manifested in faster oscillations (0.9 卤 0.3 s) in cancer cells compared to that in the normal cells (2.8 卤 0.7 s). Solvation dynamics in the lipid droplets of cancer cells is slower compared to that in the normal cell.