Integrating Histology and Imaging Mass Spectrometry

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• 作者：Pierre Chaurand ; Sarah A. Schwartz ; Dean Billheimer ; Baogang J. Xu ; Anna Crecelius ; and Richard M. Caprioli
• 刊名：Analytical Chemistry
• 出版年：2004
• 出版时间：February 15, 2004
• 年：2004
• 卷：76
• 期：4
• 页码：1145 - 1155
• 全文大小：1194K
• 年卷期：v.76,no.4(February 15, 2004)
• ISSN：1520-6882

文摘

MALDI (matrix-assisted laser desorption/ionization) imaging mass spectrometry (IMS) is a new technology thatgenerates molecular profiles and two-dimensional iondensity maps of peptide and protein signals directly fromthe surface of thin tissue sections. This allows specificinformation to be obtained on the relative abundance andspatial distribution of proteins. One important aspect ofthis is the opportunity to correlate these specific ionimages with histological features observed by opticalmicroscopy. To facilitate this, we have developed protocolsthat allow MALDI mass spectrometry imaging and opticalmicroscopy to be performed on the same section. Keycomponents of these protocols involve the use of conductive glass slides as sample support for the tissue sectionsand MS-friendly tissue staining protocols. We show theeffectiveness of these with protein standards and withseveral types of tissue sections. Although stain-specificintensity variations occur, the overall protein pattern andspectrum quality remain consistent between stained andcontrol tissue samples. Furthermore, imaging mass spectrometry experiments performed on stained sectionsshowed good image quality with minimal delocalizationof proteins resulting from the staining protocols.