Proteomics Analysis Reveals Insight into the Mechanism of H-Ras-Mediated Transformation
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- 作者：Bao-Feng Jin ; Kun He ; Hong-Xia Wang ; Bing Bai ; Tao Zhou ; Hui-Yan Li ; Jiang-Hong Man ; Bing-Yu Liu ; Wei-Li Gong ; Jie Wang ; Ai-Ling Li ; Xue-Min Zhang
- 刊名：Journal of Proteome Research
- 出版年：2006
- 出版时间：October 2006
- 年：2006
- 卷：5
- 期：10
- 页码：2815 - 2823
- 全文大小：673K
- 年卷期：v.5,no.10(October 2006)
- ISSN：1535-3907
文摘
We implemented a proteomics approach to the systematical analysis of the alterations in the proteomeof NIH3T3 cells transformed by oncogenic H-RasV12. Forty-four proteins associated with Ras-mediatedtransformation have been identified, and 28 proteins were not previously reported. RT-PCR analysisshowed that approximately 44% of target proteins identified showed concomitant changes in mRNAabundance. A principal finding was the up-regulation of gankyrin, which was the first evidence to showthat gankyrin pathway was implicated in Ras-activated transformation.