文摘
The binding behavior of substituted 1,4-bis(pyridinium)butane derivatives (X-Py(CH2)4Py-X, X = H, 2-methyl, 3-methyl, 4-methyl, 2,6-dimethyl, 4-pyridyl, and 4-COOEthyl) 12+鈥?b>72+, with negatively charged carboxylatopillar[5]arene (CP5A) has been comprehensively investigated by 1H NMR and 2D ROESY and UV absorption and fluorescence spectroscopy in aqueous phosphate buffer solution (pH 7.2). The results indicated that the position of the substituents attached on pyridinium ring dramatically affects the association constants and binding modes. 3- and 4-Substituted guests (12+, 32+, 42+, 62+, 72+) form [2]pseudorotaxane geometries with CP5A host, giving very large association constants (>105 M鈥?), while 2,6-dimethyl-substituted 52+ forms external complex with relatively small Ka values [(2.4 卤 0.3) 脳 103 M鈥?] because the 2,6-dimethylpyridinium unit is too bulky to thread the host cavity. Both of the binding geometries mentioned above are observed for 22+, having one methyl group in the 2-position of pyridinium. Typically, the association constant of [2]pseudorotaxane 12+CP5A exceeds 106 M鈥? in water, which is significantly higher than those of previously reported analogues in organic solvents. The remarkably improved complexation of bis(pyridinium) guests by the anionic host was due to electrostatic attraction forces and hydrophobic interactions.