Biocomposite Based on Reduced Graphene Oxide Film Modified with Phenothiazone and Flavin Adenine Dinucleotide-Dependent Glucose Dehydrogenase for Glucose Sensing and Biofuel Cell Applications

详细信息    查看全文

• 作者：Yehonatan Ravenna ; Lin Xia ; Jenny Gun ; Alexey A. Mikhaylov ; Alexander G. Medvedev ; Ovadia Lev ; Lital Alfonta
• 刊名：Analytical Chemistry
• 出版年：2015
• 出版时间：October 6, 2015
• 年：2015
• 卷：87
• 期：19
• 页码：9567-9571
• 全文大小：256K
• ISSN：1520-6882

文摘

A novel composite material for the encapsulation of redox enzymes was prepared. Reduced graphene oxide film with adsorbed phenothiazone was used as a highly efficient composite for electron transfer between flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenase and electrodes. Measured redox potential for glucose oxidation was lower than 0 V vs Ag/AgCl electrode. The fabricated biosensor showed high sensitivity of 42 mA M^鈥? cm^鈥?, a linear range of glucose detection of 0.5鈥?2 mM, and good reproducibility and stability as well as high selectivity for different interfering compounds. In a semibiofuel cell configuration, the hybrid film generated high power output of 345 渭W cm^鈥?. These results demonstrate a promising potential for this composition in various bioelectronic applications.