Cationic Amphiphilic Polymers with Antimicrobial Activity for Oral Care Applications: Eradication of S. mutans Biofilm

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• 作者：Haruko Takahashi ; Enrico T. Nadres ; Kenichi Kuroda
• 刊名：Biomacromolecules
• 出版年：2017
• 出版时间：January 9, 2017
• 年：2017
• 卷：18
• 期：1
• 页码：257-265
• 全文大小：514K
• ISSN：1526-4602

文摘

The antibacterial and antibiofilm activities of cationic amphiphilic methacrylate polymers against cariogenic bacterium S. mutans were investigated. Cationic homopolymer PE₀ and copolymer PE₃₁ containing 31 mol % of ethyl methacrylate were synthesized by reversible addition–fragmentation chain transfer polymerization. These polymers displayed bactericidal activity toward S. mutans and prevented biofilm formation by killing the planktonic bacteria. At a concentration of 1000 μg/mL when incubated for 2 h the polymers reduced >80% of biofilm biomass. When the polymer assay solution with the biofilm was vigorously mixed using a pipet for 30 s, >50% of biofilm mass was removed at a polymer concentration of 250 μg/mL. Chlorhexidine and a cationic surfactant failed to reduce the biofilm mass at the same concentration. PE₀ was the most effective in removing biofilm and did not show any significant cytotoxicity to human gingival fibroblast and periodontal ligament stem cells when incubated for 10 min.