Mesoporous Silica Templated Biolabels with Releasable Fluorophores for Immunoassays

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• 作者：Wenyi Cai ; Ian R. Gentle ; Gao Qing Lu ; Jun-Jie Zhu ; Aimin Yu
• 刊名：Analytical Chemistry
• 出版年：2008
• 出版时间：July 15, 2008
• 年：2008
• 卷：80
• 期：14
• 页码：5401 - 5406
• 全文大小：675K
• 年卷期：v.80,no.14(July 15, 2008)
• ISSN：1520-6882

文摘

A novel class of fluorescent immuno-biolabeling systems with extremely high F/P ratio (~1000−6000) were prepared by combining the template method and layer-by-layer (LbL) technique. Labels were constructed by loading organic dye fluorescein diacetate (FDA) molecules onto hollow periodic mesoporous organosilica (H-PMO) particles followed by ployelectrolyte encapsulation and antibody attachment. The labeling systems were stimuli-responsive to the addition of concentrated NaOH with the loaded dye molecules being released and detected in a well-controlled manner. When applied in sandwich immunoassays, results indicated that the biolabels were immuno-active and generated an optimal signal that was ~50 times higher than the conventional dye labelled antibody system.