New Class of Tetradentate 尾-Diketonate-Europium Complexes That Can Be Covalently Bound to Proteins for Time-Gated Fluorometric Application

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• 作者：Lin Zhang ; Yanjiao Wang ; Zhiqiang Ye ; Dayong Jin ; Jingli Yuan
• 刊名：Bioconjugate Chemistry
• 出版年：2012
• 出版时间：June 20, 2012
• 年：2012
• 卷：23
• 期：6
• 页码：1244-1251
• 全文大小：334K
• 年卷期：v.23,no.6(June 20, 2012)
• ISSN：1520-4812

文摘

Luminescent lanthanide complexes that can be covalently bound to proteins have shown great utility as biolabels for highly sensitive time-gated luminescence bioassays in clinical diagnostics and biotechnology discoveries. In this work, three new tetradentate 尾-diketonate鈥揺uropium complexes that can be covalently bound to proteins to display strong and long-lived Eu³⁺ luminescence, 1,2-bis[4鈥?(1鈥?1鈥?1鈥?2鈥?2鈥?3鈥?3鈥?heptafluoro-4鈥?6鈥?hexanedion-6鈥?yl)-benzyl]-4-chlorosulfobenzene-Eu³⁺ (BHHBCB-Eu³⁺), 1,2-bis[4鈥?(1鈥?1鈥?1鈥?2鈥?2鈥?pentafluoro-3鈥?5鈥?pentanedion-5鈥?yl)-benzyl]-4-chlorosulfobenzene-Eu³⁺ (BPPBCB-Eu³⁺), and 1,2-bis[4鈥?(1鈥?1鈥?1鈥?trifluoro-2鈥?4鈥?butanedion-4鈥?yl)-benzyl]-4-chlorosulfobenzene-Eu³⁺ (BTBBCB-Eu³⁺), have been designed and synthesized as biolabels for time-gated luminescence bioassay applications. The luminescence spectroscopy characterizations of the aqueous solutions of three complex-bound bovine serum albumin reveal that BHHBCB-Eu³⁺ has the strongest luminescence with the largest quantum yield (40%) and longest luminescence lifetime (0.52 ms) among the complexes, which is superior to the other currently available europium biolabels. The BHHBCB-Eu³⁺-labeled streptavidin was prepared and used for both the time-gated luminescence immunoassay of human prostate specific antigen and the time-gated luminescence microscopy imaging of a pathogenic microorganism Cryptosporidium muris. The results demonstrated the practical utility of the new Eu³⁺ complex-based biolabel for time-gated luminescence bioassay applications.