Effect of Various Modifiers on Selectivity in Packed-Column Subcritical and Supercritical Fluid Chromatography

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• 作者：Gary O. Cantrell ; Rodger W. Stringham ; John A. Blackwell ; Jeff D. Weckwerth ; and Peter W. Carr
• 刊名：Analytical Chemistry
• 出版年：1996
• 出版时间：October 15, 1996
• 年：1996
• 卷：68
• 期：20
• 页码：3645 - 3650
• 全文大小：193K
• 年卷期：v.68,no.20(October 15, 1996)
• ISSN：1520-6882

文摘

A number of common mobile phase modifiers werecharacterized by chromatographing monofunctional probesolutes on a cyano-bonded stationary phase under subcritical and supercritical conditions. Solvatochromicanalysis techniques were used to characterize the relativeselectivity, efficiency, and eluotropic strengths of thevarious modified mobile phases. Analysis of the combined results suggests that modifier choice may be madein a rational manner to either enhance or mask a particular type of molecular interaction. This control isnecessaryfor challenging applications such as chiral separations.The relative effects of each modifier property changedepending on whether the chromatographic system issubcritical or supercritical, thus making knowledge of thephase equilibria associated with the mobile phase essential.