Structural Analysis of Diheme Cytochrome c by Hydrogen鈥揇euterium Exchange Mass Spectrometry and Homology Modeling

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• 作者：Ying Zhang ; Erica L.-W. Majumder ; Hai Yue ; Robert E. Blankenship ; Michael L. Gross
• 刊名：Biochemistry
• 出版年：2014
• 出版时间：September 9, 2014
• 年：2014
• 卷：53
• 期：35
• 页码：5619-5630
• 全文大小：570K
• ISSN：1520-4995

文摘

A lack of X-ray or nuclear magnetic resonance structures of proteins inhibits their further study and characterization, motivating the development of new ways of analyzing structural information without crystal structures. The combination of hydrogen鈥揹euterium exchange mass spectrometry (HDX-MS) data in conjunction with homology modeling can provide improved structure and mechanistic predictions. Here a unique diheme cytochrome c (DHCC) protein from Heliobacterium modesticaldum is studied with both HDX and homology modeling to bring some definition of the structure of the protein and its role. Specifically, HDX data were used to guide the homology modeling to yield a more functionally relevant structural model of DHCC.