Simultaneous Quantification of Etoposide and Etoposide Phosphate in Human Plasma by Capillary Electrophoresis Using Laser-Induced Native Fluorescence Detection

详细信息    查看全文

• 作者：Uta Birgitta Soetebeer ; Marc-Oliver Schierenberg ; Harald Schulz ; Georg Hempel ; Peter Andresen ; and Gottfried Blaschke
• 刊名：Analytical Chemistry
• 出版年：2001
• 出版时间：May 15, 2001
• 年：2001
• 卷：73
• 期：10
• 页码：2178 - 2182
• 全文大小：55K
• 年卷期：v.73,no.10(May 15, 2001)
• ISSN：1520-6882

文摘

A method using capillary electrophoresis with UV laser-induced native fluorescence detection was developed asa sensitive and selective assay for the simultaneousdetermination of etoposide and etoposide phosphate inhuman plasma. Laser-induced native fluorescence detection with a frequency-doubled argon ion laser at anexcitation wavelength of 257 nm was used for the simultaneous assay of etoposide and etoposide phosphate inplasma to improve the sensitivity compared to thatobtained with UV absorption. The detection system consists of an imaging spectrograph and an intensified CCDcamera which views an illuminated 1.5-mm section of thecapillary. This setup is able to record the whole emissionspectra of the analytes to achieve additional wavelength-resolved electropherograms. In the concentration rangeof 200 mages/entities/mgr.gif">g/L-50 mg/L in plasma for etoposide and 100mages/entities/mgr.gif">g/L-20 mg/L for etoposide phosphate, coefficients ofcorrelation were better than 0.998. Within-day variationdetermined with three different concentrations showedaccuracies ranging from 91.0 to 109.3% for etoposide andfrom 91.2 to 109.9% for etoposide phosphate (n = 6) witha precision of about 8%. Day-to-day variation presentedaccuracies ranging from 91.8 to 107.9% for etoposide andfrom 94.4 to 109.3% for etoposide phosphate with arelative standard deviation less than 6% (n = 5). To ourknowledge, this is the first method for the simultaneousquantification of etoposide and etoposide phosphate inplasma samples.