Phosphate Vibrations Probe Local Electric Fields and Hydration in Biomolecules

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• 作者：Nicholas M. Levinson ; Erin E. Bolte ; Carrie S. Miller ; Steven A. Corcelli ; Steven G. Boxer
• 刊名：Journal of the American Chemical Society
• 出版年：2011
• 出版时间：August 31, 2011
• 年：2011
• 卷：133
• 期：34
• 页码：13236-13239
• 全文大小：784K
• 年卷期：v.133,no.34(August 31, 2011)
• ISSN：1520-5126

文摘

The role of electric fields in important biological processes such as binding and catalysis has been studied almost exclusively by computational methods. Experimental measurements of the local electric field in macromolecules are possible using suitably calibrated vibrational probes. Here we demonstrate that the vibrational transitions of phosphate groups are highly sensitive to an electric field and show how that sensitivity can be quantified, allowing electric field measurements to be made in phosphate-containing biological systems without chemical modification.