PTRF/Cavin-1 is Essential for Multidrug Resistance in Cancer Cells
详细信息 查看全文
- 作者：Jae-Sung Yi ; Dong-Gi Mun ; Hyun Lee ; Jun-sub Park ; Jung-Woo Lee ; Jae-Seon Lee ; Su-Jin Kim ; Bong-Rae Cho ; Sang-Won Lee ; Young-Gyu Ko
- 刊名：Journal of Proteome Research
- 出版年：2013
- 出版时间：February 1, 2013
- 年：2013
- 卷：12
- 期：2
- 页码：605-614
- 全文大小：565K
- 年卷期：v.12,no.2(February 1, 2013)
- ISSN：1535-3907
文摘
Since detergent-resistant lipid rafts play important roles in multidrug resistance (MDR), their comprehensive proteomics could provide new insights to understand the underlying molecular mechanism of MDR in cancer cells. In the present work, lipid rafts were isolated from MCF-7 and adriamycin-resistant MCF-7/ADR cells and their proteomes were analyzed by label-free quantitative proteomics. Polymerase I and transcript release factor (PTRF)/cavin-1 was measured to be upregulated along with multidrug-resistant P-glycoprotein, caveolin-1, and serum deprivation protein response/cavin-2 in the lipid rafts of MCF-7/ADR cells. PTRF knockdown led to reduction in the amount of lipid rafts on the surface of MCF7/ADR cells as determined by cellular staining with lipid raft-specific dyes such as S-laurdan2 and FITC-conjugated cholera toxin B. PTRF knockdown also reduced MDR in MCF-7/ADR cells. These data indicate that PTRF is necessary for MDR in cancer cells via the fortification of lipid rafts.