Comprehensive Two-Dimensional Liquid Chromatography Analysis of a Block Copolymer

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• 作者：Kyuhyun Im ; Hae-Woong Park ; Youngtak Kim ; Bonghoon Chung ; Moonhor Ree ; Taihyun Chang
• 刊名：Analytical Chemistry
• 出版年：2007
• 出版时间：February 1, 2007
• 年：2007
• 卷：79
• 期：3
• 页码：1067 - 1072
• 全文大小：203K
• 年卷期：v.79,no.3(February 1, 2007)
• ISSN：1520-6882

文摘

A two-dimensional liquid chromatography (2D-LC) method,normal phase liquid chromatography (NPLC) for onedimension and reversed phase liquid chromatography(RPLC) for the other dimension, was employed to mapthe molecular weight distribution (MWD) of the individualblocks of a polystyrene-block-polyisoprene (PS-b-PI)diblock copolymer. The first-dimension (1st-D) NPLCseparates PS-b-PI according to the PS block length whilethe second-dimension (2nd-D) RPLC separates PS-b-PIaccording to the PI block length. For the first-dimensionNPLC separation, the column temperature was controlledto improve the resolution while the 2nd-D RPLC was runisothermally to reduce the separation time. The MWDinformation of individual blocks provides equivalentinformation to MWD and chemical composition distribution of a block copolymer. In this analysis, the effluentfrom the 1st-D LC separation is concentrated before theinjection to the 2nd-D LC by use of a trap column, whichallows an efficient interface between the two LC separations. Over 200 different block copolymer species couldbe identified from the 2D-LC chromatogram.