Fluorescent Approach to Quantitation of Reactive Oxygen Species in Mainstream Cigarette Smoke

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• 作者：Boxin Ou and Dejian Huang
• 刊名：Analytical Chemistry
• 出版年：2006
• 出版时间：May 1, 2006
• 年：2006
• 卷：78
• 期：9
• 页码：3097 - 3103
• 全文大小：210K
• 年卷期：v.78,no.9(May 1, 2006)
• ISSN：1520-6882

文摘

A novel approach to monitoring of mainstream smokereactive oxygen species (ROS) has been developed andapplied to the quantitation of smoke oxidants. Redox-active fluorescent probe dihydrorhodamine 6G (DHR-6G)was selected as the molecular probe because it is sensitiveto typical smoke ROS. The experimental system couplesan automatic cigarette smoke machine fiber-optic fluorometer for real-time monitoring of the reaction progressbetween cigarette smoke and DHR-6G. Quantitation wasachieved based on the amount of rhodamine 6G, whichis the sole product from DHR-6G oxidation. With theoptimization of the trapping efficiency, we detected 391nmol of ROS/cigarette in the mainstream CS for a standard cigarette 2R4F under standard Federal Trade Commission smoking protocol. Applying this method, wequantified the ROS of selected cigarettes and found thatthe cigarettes made of burley tobacco have much (~10times) higher ROS content in the smoke than that in thetobacco made of bright tobacco. The smokeless cigarette,Eclipse, has comparable ROS with cigarettes made ofbright tobacco.