Detection and Relative Quantification of Proteins by Surface Enhanced Raman Using Isotopic Labels

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• 作者：Shirshendu K. Deb ; Brandon Davis ; Giselle M. Knudsen ; Ravindra Gudihal ; Dor Ben-Amotz ; V. Jo Davisson
• 刊名：Journal of the American Chemical Society
• 出版年：2008
• 出版时间：July 30, 2008
• 年：2008
• 卷：130
• 期：30
• 页码：9624-9625
• 全文大小：133K
• 年卷期：v.130,no.30(July 30, 2008)
• ISSN：1520-5126

文摘

Accurate quantification of protein content and composition has been achieved using isotope-edited surface enhanced resonance Raman spectroscopy. Synthesis of isotopomeric Rhodamine dye-linked bioconjugation reagents enabled direct labeling of surface lysines on a variety of proteins. When separated in polyacrylamide gels and stained with silver nanoparticles. The spectral signatures reflect the expected statistical distribution of isotopomeric labels on the labeled proteins in the gel matrix format without interference from protein features.