Physical Polymer Matrices Based on Affinity Interactions between Peptides and Polysaccharides

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• 作者：Brandon L. Seal and Alyssa Panitch
• 刊名：Biomacromolecules
• 出版年：2003
• 出版时间：November 2003
• 年：2003
• 卷：4
• 期：6
• 页码：1572 - 1582
• 全文大小：207K
• 年卷期：v.4,no.6(November 2003)
• ISSN：1526-4602

文摘

A rapidly forming polymer matrix with affinity-based controlled release properties was developed basedupon interactions between heparin-binding peptides and heparin. Dynamic mechanical testing of 10% (w/v)compositions consisting of a 3:1 molar ratio of poly(ethylene glycol)-co-peptide (~18 000 g/mol) to heparin(~18 000 g/mol) revealed a viscoelastic profile similar to that of concentrated, large molecular weight polymersolutions and melts. In addition, the biopolymer mixtures recovered quickly following thermal denaturationand mechanical insult. These gel-like materials were able to sequester exogenous heparin-binding peptidesand could release these peptides over several days at rates dependent on relative heparin affinity. The initialrelease rates ranged from 3.3% per hour for a peptide with low heparin affinity to 0.025% per hour for apeptide with strong heparin affinity. By altering the affinity of peptides to heparin, a series of peptides canbe developed to yield a range of release profiles useful for controlled in vivo delivery of therapeutics.