Engineering Photosystem I Complexes with Metal Oxide Binding Peptides for Bioelectronic Applications

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• 作者：Richard F. Simmerman ; Tuo Zhu ; David R. Baker ; Lijia Wang ; Sanjay R. Mishra ; Cynthia A. Lundgren ; Barry D. Bruce
• 刊名：Bioconjugate Chemistry
• 出版年：2015
• 出版时间：October 21, 2015
• 年：2015
• 卷：26
• 期：10
• 页码：2097-2105
• 全文大小：490K
• ISSN：1520-4812

文摘

Conventional dye-sensitized solar cells comprise semiconducting anodes sensitized with complex synthetic organometallic dyes, a platinum counter electrode, and a liquid electrolyte. This work focuses on replacing synthetic dyes with a naturally occurring biological pigment鈥損rotein complex known as Photosystem I (PSI). Specifically, ZnO binding peptides (ZOBiP)-fused PSI subunits (ZOBiP鈥揚saD and ZOBiP鈥揚saE) and TiO<sub>2sub> binding peptides (TOBiP)-fused ferredoxin (TOBiP鈥揊d) have been produced recombinantly from Escherichia coli. The MOBiP-fused peptides have been characterized via western blotting, circular dichroism, MALDI-TOF, and cyclic voltammetry. ZOBiP鈥揚SI subunits have been used to replace wild-type PsaD and PsaE, and TOBiP鈥揊d has been chemically cross-linked to the stromal hump of PSI. These MOBiP peptides and MOBiP鈥揚SI complexes have been produced and incubated with various metal oxide nanoparticles, showing increased binding when compared to that of wild-type PSI complexes.