Selective Quantification by 2D HSQC0 Spectroscopy of Thiocoraline in an Extract from a Sponge-Derived Verrucosispora sp.

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• 作者：Kaifeng Hu ; Thomas P. Wyche ; Tim S. Bugni ; John L. Markley
• 刊名：Journal of Natural Products
• 出版年：2011
• 出版时间：October 28, 2011
• 年：2011
• 卷：74
• 期：10
• 页码：2295-2298
• 全文大小：750K
• 年卷期：v.74,no.10(October 28, 2011)
• ISSN：1520-6025

文摘

We recently developed a 2D <sup>1sup>H鈥?sup>13sup>C HSQC<sub>0sub> approach to quantify individual chemicals in complex mixtures. The HSQC<sub>0sub> approach has been implemented in phase-cycled and gradient-selective versions. As in quantitative 1D NMR, the normalized integrated signal intensities in HSQC<sub>0sub> are proportional to the concentrations of individual chemicals in the mixture. We applied the HSQC<sub>0sub> approach to selectively quantify thiocoraline present at a level of 1% w/w in an extract from a Verrucosispora sp. isolated from the sponge Chondrilla caribensis f. caribensis. We expect that this approach can be used to quantify other natural products of interest in extracts without prior purification.