The Use of Deuterium Oxide as a Mobile Phase for Structural Elucidation by HPLC/UV/ESI/MS
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- 作者：Mark A. Olsen ; Paul G. Cummings ; Sonya Kennedy-Gabb ; Brian M. Wagner ; Gordon R. Nicol ; and Burnaby Munson
- 刊名：Analytical Chemistry
- 出版年：2000
- 出版时间：October 15, 2000
- 年：2000
- 卷：72
- 期：20
- 页码：5070 - 5078
- 全文大小：168K
- 年卷期：v.72,no.20(October 15, 2000)
- ISSN：1520-6882
文摘
The use of deuterium oxide as a mobile phase in theroutine analysis of pharmaceutical compounds was investigated. The deuterium exchange of labile hydrogenatoms aids in structural confirmation and elucidation ofunknown impurities and degradation products. Althoughdeuterium oxide as a mobile phase does in some caseschange the retention times, the changes in retention timesdo not interfere with the analysis. A study of the high-performance liquid chromatography system shows thatequilibration times for the deuterium-containing mobilephases are similar to equilibration times with changes ofother mobile phases. The use of this technique in theanalysis of pharmaceutical compounds and other smallmolecules is presented.