Antioxidant Activity and Protective Effect of Anthocyanin Oligomers on H2O2-Triggered G2/M Arrest in Retinal Cells

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• 作者：Jin-Woo Hwang ; Eun-Kyung Kim ; Seung-Jae Lee ; Yon-Suk Kim ; Sang-Ho Moon ; Byong-Tae Jeon ; Si-Heung Sung ; Eun-Tae Kim ; Pyo-Jam Park
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2012
• 出版时间：May 2, 2012
• 年：2012
• 卷：60
• 期：17
• 页码：4282-4288
• 全文大小：343K
• 年卷期：v.60,no.17(May 2, 2012)
• ISSN：1520-5118

文摘

In this study, the free-radical-scavenging properties of anthocyanin oligomers for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, alkyl radical, and hydroxyl radical were evaluated using electron spin resonance (ESR) spectroscopy. The DPPH radical, alkyl radical, and hydroxyl radical scavenging activity of anthocyanin oligomers increased in a dose-dependent manner, with the 50% inhibitory concentration (IC₅₀) value of 13.0, 14.0, and 448.0 渭g/mL, respectively. The inhibitory effect of anthocyanin oligomers on lipid peroxidation was examined with ferric thiocyanate (FTC) and thiobarbituric acid (TBA). The inhibitory activity of anthocyanin oligomers was found to be comparable to that of vitamin E. In addition, anthocyanin oligomers enhanced the activities of superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6), glutathione peroxidase (GPx, EC 1.11.1.9), and glutathione-S-transferase (GST, EC 2.5.1.18) in ARPE-19 cells. In addition, anthocyanin oligomers inhibited the H₂O₂-induced G2/M phase arrest in ARPE-19 cells. Taken together, the present results demonstrate that anthocyanin oligomers have high antioxidative activity.

Keywords:

antioxidation; anthocyanin oligomers; ARPE-19; G2/M phase arrest