Reactive Oxygen Scavenging Effect of Enzymatic Extracts from Sargassum thunbergii

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• 作者：Pyo-Jam Park ; Soo-Jin Heo ; Eun-Ju Park ; Se-Kwon Kim ; Hee-Guk Byun ; Byong-Tae Jeon ; and You-Jin Jeon
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2005
• 出版时间：August 24, 2005
• 年：2005
• 卷：53
• 期：17
• 页码：6666 - 6672
• 全文大小：219K
• 年卷期：v.53,no.17(August 24, 2005)
• ISSN：1520-5118

文摘

The free radical scavenging activity of water soluble natural antioxidants from Sargassum thunbergii,which is a brown marine alga, was evaluated by examining the radical scavenging activities of theextracts of hydrolyzates from S. thunbergii on hydroxyl, 1,1-diphenyl-2-picrylhydrazyl (DPPH), andalkyl radicals. A spin-trapping electron spin resonance (ESR) spectrometer was employed, and theresults were compared for their ESR signal intensity. S. thunbergii was enzymatically hydrolyzed toprepare water soluble extracts by five carbohydrases (AMG, Celluclast, Termamyl, Ultraflo, andViscozyme) and proteases (Alcalase, Flavorzyme, Kojizyme, Neutrase, and Protamex). The scavenging activity of the radicals increased with increased concentrations of the extracts. The scavengingresults were higher for hydroxyl and alkyl radicals and lower for DPPH radical as compared withvitamin C as a reference. The hydrogen peroxide scavenging activity of the extracts was alsoinvestigated; the Alcalase extract showed the highest scavenging activity among the extracts preparedwith the five proteases and five carbohydrates. In addition, the DNA damage was determined byusing the comet assay with alkaline electrophoresis and was quantified by measuring the tail length.The preventive effect of Alcalase extract from S. thunbergii against DNA damage increased withincrements of concentration of the enzymatic extracts.Keywords: Enzymatic extract; Sargassum thunbergii; marine alga; electron spin resonance; radicalscavenging activity; comet assay