Seeking Transformation Markers: An Analysis of Differential Tissue Proteomes on the Rice Germplasm Generated from Transformation of Echinochloa crusgalli Genomic DNA
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- 作者：Caifeng Zhao ; Bingran Zhao ; Yan Ren ; Wei Tong ; Jingqiang Wang ; Kang Zhao ; Shaokun Shu ; Ningzhi Xu ; Siqi Liu
- 刊名：Journal of Proteome Research
- 出版年：2007
- 出版时间：April 2007
- 年：2007
- 卷：6
- 期：4
- 页码：1354 - 1363
- 全文大小：504K
- 年卷期：v.6,no.4(April 2007)
- ISSN：1535-3907
文摘
The transformation of distally related genomic DNAs into plant was proposed as a novel technique tobreed new cultivars. For example, a restorer rice line, RB207, was successfully developed and stabilizedthrough the transformation of genomic DNAs of Echinochloa crusgalli (E. crusgalli) into a rice line,R207. Although the phenotypes of this variant line are apparently different from its receptor, themolecular bases are not elucidated yet. Herein, we have systematically studied the differential proteomesfrom the tissues of E. crusgalli, R207, and RB207 in an attempt to find an explanation regarding thephenotypic changes of RB207. The 2-DE method was employed to separate the leaf and embryo proteinsof these plants followed by protein identification with mass spectrometry. In the leaf, 953 ± 15, 1084± 11, and 1091 ± 11 silver-stained spots were detected, whereas in the embryo, 986 ± 3, 884 ± 10, and892 ± 14 spots were found from E. crusgalli, R207, and RB207, respectively. In comparison to the 2-DEimages of the two rice lines, which showed many similarities, the ones of the E. crusgalli and rice werefound to be so different that they were incomparable. There were some differentially expressed 2-DEspots between the two rice cultivars, 72 in leaf and 53 in embryo, respectively. The results of proteinidentification suggested that, regardless of leaves or embryos, none of the E. crusgalli genes wereencoded in the new rice cultivar, RB207. The fact that 60% of the differentially expressed spots betweenR207 and RB207, however, were verified as the proteins involved in metabolism and photosynthesismakes a rather convincing argument that the DNA fragments transferred from E. crusgalli to rice areresponsible for exerting the unknown influence to the expression of rice genes.