Carbon Nanodots As a Matrix for the Analysis of Low-Molecular-Weight Molecules in Both Positive- and Negative-Ion Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry and Quant
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- 作者：Suming Chen ; Huzhi Zheng ; Jianing Wang ; Jian Hou ; Qing He ; Huihui Liu ; Caiqiao Xiong ; Xianglei Kong ; Zongxiu Nie
- 刊名：Analytical Chemistry
- 出版年：2013
- 出版时间：July 16, 2013
- 年：2013
- 卷：85
- 期：14
- 页码：6646-6652
- 全文大小：305K
- 年卷期：v.85,no.14(July 16, 2013)
- ISSN：1520-6882
文摘
Carbon nanodots were applied for the first time as a new matrix for the analysis of low-molecular-weight compounds by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in both positive- and negative-ion modes. A wide range of small molecules including amino acids, peptides, fatty acids, as well as 尾-agonists and neutral oligosaccharides were analyzed by MALDI MS with carbon nanodots as the matrix, and the lowest 0.2 fmol limits-of-detection were obtained for octadecanoic acid. Clear sodium and potassium adducts and deprotonated signals were produced in positive- and negative-ion modes. Furthermore, the glucose and uric acid in real samples were quantitatively determined by the internal standard method with the linear range of 0.5鈥? mM and 0.1鈥?.8 mM (R2 > 0.999), respectively. This work gives new insight into the application of carbon nanodots and provides a general approach for rapid analysis of low-molecular-weight compounds.