Development of a Broad-Specificity Monoclonal Antibody-Based Immunoaffinity Chromatography Cleanup for Organophosphorus Pesticide Determination in Environmental Samples

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• 作者：Zhen-Lin Xu ; Hao Deng ; Hong-Tao Lei ; Yue-Ming Jiang ; Katrina Campbell ; Yu-Dong Shen ; Jin-Yi Yang ; Hong Wang ; Yuan-Ming Sun
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2012
• 出版时间：June 13, 2012
• 年：2012
• 卷：60
• 期：23
• 页码：5847-5852
• 全文大小：239K
• 年卷期：v.60,no.23(June 13, 2012)
• ISSN：1520-5118

文摘

An immunoaffinity chromatographic (IAC) method for the selective extraction and concentration of 13 organophosphorus pesticides (OPs, including coumaphos, parathion, phoxim, quinalphos, dichlofenthion, triazophos, azinphos-ethyl, phosalone, isochlorthion, parathion-methyl, cyanophos, disulfoton, and phorate) prior to analysis by high-performance liquid chromatography鈥搕andem mass spectrometry (HPLC鈥揗S/MS) was developed. The IAC column was prepared by covalently immobilizing a monoclonal antibody with broad specificity for OPs on CNBr-activated Sephrose 4B. The column capacity ranged from 884 to 2641 ng/mL of gel. The optimum elution solvent was 0.01 M phosphate-buffered saline containing 80% methanol. The breakthrough volume of the IAC column was found to be 400 mL. Recoveries of OPs from spiked environmental samples by IAC cleanup and HPLC鈥揗S/MS analysis ranged from 60.2 to 107.1%, with a relative standard deviation below 11.1%. The limit of quantitation for 13 OPs ranged from 0.01 to 0.13 ng/mL (ng/g). The application of IAC cleanup coupled to HPLC鈥揗S/MS in real environmental samples demonstrated the potential of this method for the determination of OP residues in environmental samples at trace levels.
<h4>Keywords: h4> organophosphorus pesticides; immunoaffinity chromatography; HPLC鈭扢S/MS; environmental samples