Screening and Selection of High Carotenoid Producing in Vitro Tomato Cell Culture Lines for [13C]-Carotenoid Production

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• 作者：Nancy J. Engelmann ; Jessica K. Campbell ; Randy B. Rogers ; S. Indumathie Rupassara ; Peter J. Garlick ; Mary Ann Lila ; John W. Erdman ; Jr.
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2010
• 出版时间：September 22, 2010
• 年：2010
• 卷：58
• 期：18
• 页码：9979-9987
• 全文大小：911K
• 年卷期：v.58,no.18(September 22, 2010)
• ISSN：1520-5118

文摘

Isotopically labeled tomato carotenoids, phytoene, phytofluene, and lycopene, are needed for mammalian bioavailability and metabolism research but are currently commercially unavailable. The goals of this work were to establish and screen multiple in vitro tomato cell lines for carotenoid production, test the best producers with or without the bleaching herbicides, norflurazon and 2-(4-chlorophenyl-thio)triethylamine (CPTA), and to use the greatest carotenoid accumulator for in vitro ¹³C-labeling. Different Solanum lycopersicum allelic variants for high lycopene and varying herbicide treatments were compared for carotenoid accumulation in callus and suspension culture, and cell suspension cultures of the hp-1 line were chosen for isotopic labeling. When grown with [U]-¹³C-glucose and treated with CPTA, hp-1 suspensions yielded highly enriched ¹³C-lycopene with 45% of lycopene in the M+40 form and 88% in the M+35 to M+40 isotopomer range. To the authors’ knowledge this is the first report of highly enriched ¹³C-carotenoid production from in vitro plant cell culture.

Keywords:

Solanum lycopersicum; Solanum pimpinellifolium; plant cell culture; lycopene; phytoene; metabolic tracing; isotopic labeling; carotenoids; tomato; nutrition; metabolism