Ion Mobility Mass Spectrometry of Peptide Ions: Effects of Drift Gas and Calibration Strategies

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• 作者：Matthew F. Bush ; Iain D. G. Campuzano ; Carol V. Robinson
• 刊名：Analytical Chemistry
• 出版年：2012
• 出版时间：August 21, 2012
• 年：2012
• 卷：84
• 期：16
• 页码：7124-7130
• 全文大小：415K
• 年卷期：v.84,no.16(August 21, 2012)
• ISSN：1520-6882

文摘

One difficulty in using ion mobility (IM) mass spectrometry (MS) to improve the specificity of peptide ion assignments is that IM separations are performed using a range of pressures, gas compositions, temperatures, and modes of separation, which makes it challenging to rapidly extract accurate shape parameters. We report collision cross section values (惟) in both He and N₂ gases for 113 peptide ions determined directly from drift times measured in a low-pressure, ambient temperature drift cell with radio-frequency (rf) ion confinement. These peptide ions have masses ranging from 231 to 2969 Da, 惟_He of 89鈥?16 脜², and 惟_N2 of 151鈥?01 脜²; thus, they are ideal for calibrating results from proteomics experiments. These results were used to quantify the errors associated with traveling-wave 惟 measurements of peptide ions and the errors concomitant with using drift times measured in N₂ gas to estimate 惟_He. More broadly, these results enable the rapid and accurate determination of calibrated 惟 for peptide ions, which could be used as an additional parameter to increase the specificity of assignments in proteomics experiments.