Smartphone-Based Simultaneous pH and Nitrite Colorimetric Determination for Paper Microfluidic Devices

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• 作者：Nuria Lopez-Ruiz ; Vincenzo F. Curto ; Miguel M. Erenas ; Fernando Benito-Lopez ; Dermot Diamond ; Alberto J. Palma ; Luis F. Capitan-Vallvey
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：October 7, 2014
• 年：2014
• 卷：86
• 期：19
• 页码：9554-9562
• 全文大小：410K
• ISSN：1520-6882

文摘

In this work, an Android application for measurement of nitrite concentration and pH determination in combination with a low-cost paper-based microfluidic device is presented. The application uses seven sensing areas, containing the corresponding immobilized reagents, to produce selective color changes when a sample solution is placed in the sampling area. Under controlled conditions of light, using the flash of the smartphone as a light source, the image captured with the built-in camera is processed using a customized algorithm for multidetection of the colored sensing areas. The developed image-processing allows reducing the influence of the light source and the positioning of the microfluidic device in the picture. Then, the H (hue) and S (saturation) coordinates of the HSV color space are extracted and related to pH and nitrite concentration, respectively. A complete characterization of the sensing elements has been carried out as well as a full description of the image analysis for detection. The results show good use of a mobile phone as an analytical instrument. For the pH, the resolution obtained is 0.04 units of pH, 0.09 of accuracy, and a mean squared error of 0.167. With regard to nitrite, 0.51% at 4.0 mg L^鈥? of resolution and 0.52 mg L^鈥? as the limit of detection was achieved.